The proposal for a bar, nightclub and conference centre within a landmark public park is now facing opposition from two sets of politicians and at the Scottish Parliament.
Five Labour councillors and a separate cross-party group want Glasgow City Council's recently established scrutiny committees to re-examine the bid by Stefan King's G1 Group for the scheme in the west end's Botanic Gardens.
The plans could ultimately be referred back to the authority's executive committee and the original decision overturned.
It comes as a number of Glasgow Nationalist MSPs submitted a motion at parliament demanding a reversal of last week's decision to agree in principle to the application.
One "call-in" to the scrutiny committee came from its convener, Labour's Euan McLeod, backed by party colleagues Paul Rooney, Paul Carey, Phil Braat and Tommy Morrison.
But as Mr McLeod's was submitted first only his will be heard, although he insists that local opposition councillors along with other interested stakeholders will be given the chance to speak at the meeting which is scheduled to take place in the late summer or early autumn.
However, opposition members are concerned that the move may be a pre-emptive strike by Labour to ensure the scrutiny is less rigorous than if they had conducted it.
West end SNP member Alex Dingwall said: "I hope this signals a re-think within the Labour group and isn't some sort of squalid tactic."
Yesterday, SNP MSPs Bob Doris, Bill Kidd and Sandra White lodged a motion "calling on Glasgow City Council to halt any further moves on this decision and enter into a full consultation exercise with local communities and other interested groups".
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